http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114509513-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e56dfe1af97a8ce2f544fd7a44b7e684 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-045 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 |
| filingDate | 2021-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89c3e15934ec9639e19015f021de1824 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2775d97f1108c4c9056802b14e1af844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68a9f90d65e243c1eeda2e44acebbed1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b8624c29d4d933d6ca0a4b2da80c59c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58bc765f84d8b7c980838c3622650e50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cbdeb9350a99049839a0410d1e2a4452 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f689256336d4dbeafcfe89713c765a8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ca10b2902ca30d42ee9951b4bfc5a59 |
| publicationDate | 2022-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114509513-A |
| titleOfInvention | Qualitative and quantitative detection method of bile acids in multiple tissues by liquid chromatography and high-resolution mass spectrometry |
| abstract | The invention belongs to the field of drug analysis, in particular to a method for qualitative and quantitative detection of bile acids in multiple tissues by liquid chromatography and high-resolution mass spectrometry. The detection method includes the following steps: firstly preparing a mixed internal standard working solution and a standard solution, pre-processing the bile acid sample to be measured, and then performing liquid chromatography mass spectrometry analysis, wherein in the liquid chromatography conditions, the mobile phase A is 0.05-0.2 % formic acid water, mobile phase B is 0.05-0.2% formic acid acetonitrile, gradient elution is adopted, the flow rate is 350 μl/min, and the column temperature is 35-45 °C. The conditions of mass spectrometry analysis were as follows: electrospray ion source, negative ion scanning, nebulizer pressure 48‑52psi; collision energy 58‑62V; capillary voltage 5.2‑5.7kV; ion temperature 590‑610℃; mass range m/z 50‑ 800Da. The invention establishes a qualitative and quantitative detection method for bile acids in various tissues, which can realize the qualitative and quantitative detection of various types of bile acids in various tissues of animals such as fish, mice, or medicinal materials such as Dannanxing, and has the advantages of simple method and reagents. Simple and sensitive detection. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116183780-A |
| priorityDate | 2021-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 105.