http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114507658-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2800-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23V2002-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K8-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-7012 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-03003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23L33-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61Q19-00 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L33-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61Q19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K8-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-7012 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2022-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114507658-B |
| titleOfInvention | Enzyme co-expression system and its application in the synthesis of sialic acid |
| abstract | The present invention relates to the field of biochemistry, in particular to an enzyme co-expression system and its application in synthesizing sialic acid. The present invention provides an N-acetylneuraminic acid aldolase (NAL) mutant having the amino acid sequence shown in SEQ ID NO: 1; or a substitution, deletion, addition and/or substitution 1 on the basis of the above amino acid sequence A sequence of one or more amino acids; or a sequence with more than 90% homology to the above amino acid sequence. The dual-promoter co-expression system adopted in the present invention simultaneously adopts the molecular biological means of increasing expression, realizes the simultaneous overexpression of two enzymes, and realizes the preparation of enzyme liquid by one-time sterilization process, thereby improving the reaction efficiency of the enzyme-catalyzed reaction. . At the same time, the NAL of Clostridium scindens was modified to improve the synthesis efficiency of sialic acid produced by the forward reaction. The yield of the enzyme-catalyzed process in the present invention meets the requirements of industrial production. |
| priorityDate | 2022-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 669.