| abstract |
The invention belongs to the technical field of avian disease detection, and in particular relates to a patent application for an ELISA detection method of avian-derived exosomes. The ELISA detection method for avian-derived exosomes includes the steps of sample preparation, coating, sealing, sample addition, detection, and result determination. In the prior art, the ELISA detection methods based on exosomes all target human exosome surface marker proteins CD9 and CD81, but since these marker proteins are not suitable for poultry use, that is, they cannot be used to detect poultry origin. Exosome detection. To this end, this application uses the avian-derived exosome surface marker protein CD63 as a marker, by preparing avian-derived CD63 protein and preparing its corresponding multi-antibody serum, and further based on this, an ELISA for avian-derived exosomes is designed and developed. The detection method can then lay a certain technical foundation for the research on poultry-related physiological work. |