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filingDate 2020-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2022-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114480514-A
titleOfInvention Method for producing pyrogallic acid by fermentation of Streptococcus thermophilus
abstract The invention belongs to the field of microbial fermentation engineering, and mainly introduces a method for producing pyrogallic acid by Streptococcus thermophilus. The Streptococcus thermophilus was inserted into the medium, and the composition and content of the medium were: peptone 7.5g/L, yeast extract 5g/L, glucose 8.5g/L, Tween 80 0.5ml/L, pH 6.0-7.0 , After culturing at 37°C for 16-24h, the cells were collected by centrifugation, washed with physiological saline, and centrifuged again. The obtained cells were dispersed in the transformation medium, and the sterile substrate tannic acid was added to make the final concentration 40mg/ml, and allowed to stand at 37°C. Transformation 6h. The transformation solution was extracted with ethyl acetate, concentrated to obtain the extract, and the extract was purified by silica gel column to obtain pyrogallic acid. The method utilizes facultative anaerobic bacteria for transformation, does not need anaerobic environment, has mild process reaction conditions, simple operation, good reproducibility, low cost, short time, and is environmentally friendly to equipment and the like.
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