http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114480470-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f1d2cfabe8bf9f1c9c2a9a4b86d7869c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-905 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07049 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1276 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 |
| filingDate | 2020-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a452f76e9fc3455b7e5da06f4199ea5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_151fa1e5b8c9f0c5acd3836c1de257e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54451eb95fe5358656687c906a81f60a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0f4b4d72cb1452a19ff9c48bb219c1e |
| publicationDate | 2022-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114480470-A |
| titleOfInvention | Methods for high-throughput preparation of gene editing mutants in model organisms and related plasmids |
| abstract | The present invention provides a high-throughput method for preparing gene editing mutants of model organisms and related plasmids. Wherein, the method includes: using a cell of a model organism capable of expressing Cas protein and reverse transcriptase as a test starting cell; introducing a knock-in fragment targeting a target gene into the test starting cell, and the knock-in fragment includes a gene with a selectable marker The homologous recombination repairs the template sequence and the DNA sequence of the guide RNA to obtain gene-editable cells; make the gene-editable cells initiate gene editing and screen the corresponding phenotype of the selectable marker gene to obtain the gene whose target gene is replaced by the selectable marker gene Edit mutants. This method not only makes full use of the advantages of high-efficiency and high-throughput gene editing to generate mutants, but also uses screening marker genes to quickly and efficiently screen and isolate target mutants, which overcomes the cumbersome and time-consuming problems of existing methods. It lays the foundation for large-scale or high-throughput production of gene knockout mutants. |
| priorityDate | 2020-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 88.