http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114480254-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_240bb64e81e2404c8034f687593869c1 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-067 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5014 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2022-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4189e851de9ad44e2d0227a252150bc6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_158f8594cf0376df8cc0e6b0eafeb76a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f70543d31448aaa01055a5c57ed038b6 |
| publicationDate | 2022-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114480254-A |
| titleOfInvention | In vitro surrogate model of hepatotoxicity constructed by HepaRG and HepG2 and its construction method and application |
| abstract | The invention discloses an in vitro hepatotoxicity surrogate model constructed by HepaRG and HepG2 and a construction method thereof. The model is obtained by culturing and differentiating two kinds of human hepatocytes under conventional conditions. After differentiation, HepaRG cells have enhanced metabolic capacity and increased sensitivity to compounds. The established model can be used to detect a variety of hepatotoxic compounds, and can also be used to explore the toxicity mechanism of known hepatotoxic drugs. After in vitro verification, it is shown that the hepatotoxicity markers screened by the present invention are suitable for the evaluation of the hepatotoxicity of food contaminants, and their expression levels can be used for the classification of hepatotoxicity grades. The in vitro hepatotoxicity surrogate model constructed by HepaRG and HepG2 of the present invention can be applied in the safety evaluation of hepatotoxic drugs. Compared with the traditional liver function index, the measurement result of the invention is more stable, and has been verified by animal experiments, which makes up the blank of the previous in vitro research on the hepatotoxicity of food contaminants. |
| priorityDate | 2022-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.