| abstract |
The invention provides a use of Pinctada martensii mucilage for extracting exosomes, exosomes, and an extraction method and application thereof. The invention adopts ultracentrifugation to separate vesicle-like nanoparticles from the mucus of Pinctada martensii, and identify them as exosomes, thereby providing a reference for extracting biologically active exosomes from marine organisms, and at the same time improving the Utilization value of Pinctada martensii. The exosomes extracted from Pinctada martensii mucus in the present invention can enhance cell viability, reduce intracellular ROS level, inhibit the activation of NF-κB and NLRP3 pathways, reduce the mRNA expression level of related inflammatory factors, and have certain anti-inflammatory properties. effect. |