abstract |
The invention provides an ENO2 monoclonal antibody, a preparation method and application thereof, and relates to the technical field of monoclonal antibodies. The present invention provides the sequences of the complementarity determining regions of the variable regions of the heavy chain and the light chain of the monoclonal antibody 11D7. Based on the sequences, the variable region sequences can be constructed on an antibody expression vector, and expressed in transfected cells to obtain a recombinant monoclonal antibody. cloned antibodies. The monoclonal antibody carrier obtained by the present invention is easy to store and easy to control the quality of the antibody production process; it is also convenient to do a series of transformations on the antibody, for more in-depth research and wider application. |