| abstract |
The invention discloses a preparation method and application of a collagen scaffold for endometrial repair combined with umbilical cord stem cells. The human umbilical cord mesenchymal stem cells are firstly obtained, wherein the human umbilical cord mesenchymal stem cells are taken out from a refrigerator and thawed, and then Inoculated into a culture flask, and then prepared a single-cell suspension, wherein the human umbilical cord mesenchymal stem cells cultivated in the culture flask were passaged, and then the UC-MSCs were trypsinized to prepare a single-cell suspension, followed by compound culture, that is, The single-cell suspension is evenly added dropwise to the collagen scaffold to prepare the collagen scaffold for endometrial repair combined with umbilical cord stem cells, and finally the subsequent culture is carried out. In the present invention, human umbilical cord mesenchymal stem cells are used for mixed culture on collagen scaffolds, and the collagen scaffolds that have been approved by SFDA are used for endometrial repair technology through relatively simple technology, which has good safety and is beneficial for stem cells to treat patients with severe endometrial damage. scientific research. |