http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114460303-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0d356f4c53d45fec26a20a6ae07902fc |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-44 |
| filingDate | 2021-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5fce97ad096dc48f6b1d0c41ad4e17f0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60dcf9a95467f32f32bd0809f5d6d5e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33675334f36b71dca881f6a5bf74ee51 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd416444d89f9dca9dbe6d2e7e3d7172 |
| publicationDate | 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114460303-A |
| titleOfInvention | A method for the interaction of CsENO2 protein of Isospora suis with unknown proteins in host cells |
| abstract | The invention discloses a method for the interaction between the Isospora suis CsENO2 protein and an unknown protein of a host cell. The method uses Enos primers and pET30(a)‑enolase as a template to carry out PCR reaction to obtain the Isospora suis CsENO2 gene, and then the Isospora suis CsENO2 gene is cloned into eukaryotic expression On the vector pcDNA3.1, and transfected into the cell, after expressing the CsENO2 protein through eukaryotes, the CsENO2 antibody was used to capture the intracellular interacting protein by co-immunoprecipitation, and finally increased by SDS-PAGE analysis. The identity of the interacting protein can be confirmed after the protein band is analyzed by mass spectrometer. The technical threshold of the method of the present invention is relatively low, and only basic transfection technology and co-immunoprecipitation technology can be analyzed; meanwhile, the present invention is not prone to the problem of false positives. |
| priorityDate | 2021-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID414886086 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID19732 |
Total number of triples: 22.