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filingDate 2022-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0fb42fef2fcea48e3d7baeb9398df15
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publicationDate 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114460297-A
titleOfInvention An immunofluorescence kit for detecting circulating prostate epithelial cells in blood and method of using the same
abstract The invention discloses an immunofluorescence kit for detecting circulating prostate epithelial cells in blood and a using method thereof, belonging to the technical field of molecular biology. The immunofluorescence kit for detecting circulating prostate epithelial cells in blood includes a fixative solution, a blocking solution, a cell membrane permeabilization solution, an antibody staining solution, a fluorescent dye, a DAPI staining solution and a mounting solution. Wherein, the antibody staining solution is a mixed solution of mouse anti-CK15 antibody and rat anti-CD45 antibody. The invention also discloses a method of using the above-mentioned immunofluorescence kit for detecting circulating prostate epithelial cells in blood. The immunofluorescence kit of the invention adopts the combination of mouse anti-CK15 antibody and rat anti-CD45 antibody, which can screen out the prostate epithelial cells free in the blood with high specificity, and has a high detection rate of benign prostatic hyperplasia and prostate cancer. .
priorityDate 2022-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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