| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a582dfa94e7141cbfd492947bf75f6b |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23K50-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23K10-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2414 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23K10-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23K50-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate |
2021-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a11f9cdab3b76b0c73d6678b6009223
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbb789b3819c56ed4c32ee9c59b09039
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dab8b6cd05f6fbc8c1e9440e307d4e86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ea4a8ea4917ff569893c85de7b3a53b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_628e3f18c589241f287eb26f98a7c796
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a8ffc26a8f8ba6e62d2a8334b26bfda1 |
| publicationDate |
2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-114457058-A |
| titleOfInvention |
A kind of mutation improvement method and application of forage alpha amylase |
| abstract |
The present invention discloses L-type alpha amylase variant and its application. The α-amylase variant is an α-amylase that sequentially mutates four amino acid residues in the amino acid sequence of the parent α-amylase, and still retains the ability of the parent to hydrolyze the α-1,4 glycosidic bond. The α-amylase variant provided by the invention has higher catalytic activity under the acidic conditions of 80°C to 100°C and pH 2.5 to 5.5 for 2 hours and under high temperature conditions above 90°C. Amylase is the key enzyme in starch digestion. Adding amylase to feed can improve the utilization rate of feed. The α-amylase mutant provided by the present invention is better than in vitro enzymatic hydrolysis of laying hen feed (starch content up to 57%) at pH 2.7 and temperature of 41° C. under different enzyme dosage, enzymatic hydrolysis time and solid-liquid ratio. native amylase. The α-amylase mutant provided by the invention has good acid resistance, thermal stability and excellent feed enzymolysis effect, and can be applied to the animal feed industry. |
| priorityDate |
2021-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |