| abstract |
The invention belongs to the technical field of cell biology, and proposes a method for inducing pluripotent stem cells into neural progenitor cells for culturing and expanding and application thereof. /F12 medium is diluted to make Matrigel coating liquid, and the Matrigel coating liquid is pre-cooled; S2: The pre-cooled Matrigel coating liquid is transferred to a petri dish, and placed in an incubator to stand, to obtain Matrigel coating Petri dish; S3: NPC cells with a degree of confluence reaching 70-80%, washed with PBS, and then digested with Accutase digestion enzyme; S4: centrifuged the cells in the centrifuge tube to remove the supernatant, adopt NPC maintenance medium and add ROCK Inhibitor resuspended NPC cells and seeded them in Matrigel-coated dishes for culture; S5: 1 day later, aspirate the liquid in Matrigel-coated dishes, and observe the morphology of NPC cells. The neural progenitor cells of the present invention are derived from the directional differentiation of induced human pluripotent stem cells, have the shape and characteristics of human neural progenitor cells, and realize long-term maintenance and culture of the cells. |