| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a8e25a7e94765d9da247a661ac56106 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-55 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-468
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-46
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-46 |
| filingDate |
2020-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de1d0537e17c6e35a90c5b3fc04e7f51
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13a42490e8ed054b09f8c4d118aabc0d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9378ec8d2b7241fc8cc0f42a90634d31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_926ea19ae7df81666da20198ac56f2d3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8194153301ba40e56e0c567e5e8e0f15 |
| publicationDate |
2022-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-114437226-A |
| titleOfInvention |
Methods of making bispecific antibodies |
| abstract |
The present invention provides a method for improving the correct pairing rate of heavy and light chains during the preparation of bispecific antibodies. The method includes eliminating natural interchain disulfides by amino acid substitution on the CH1-CL domain of one Fab arm. bond, while forming an engineered interchain disulfide bond by amino acid substitution. The method of the present invention further comprises the step of reversing the charge of a pair of amino acids of the CH1-CL domain of the Fab arm by amino acid substitution. The method of the present invention can significantly improve the rate of correct pairing of heavy and light chains in bispecific antibody production, and is applicable to various antibody types. |
| priorityDate |
2020-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |