http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114410844-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efe2ce56eb6579b8e37719a7e58ef3f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_13ad9024111e372da5ed51fd0c55c74d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-702 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2022-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5d24e0f892c0307bb8e584bddabe48a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f42d3823c4b4d32bb16f4a2f677f7dc4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9097f19132ea9641b2afddfd6fb4225b |
| publicationDate | 2022-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114410844-A |
| titleOfInvention | Method for determining RCR negative and positive in CAR-T cell product by detecting GALV and ALB copy number through multiple qPCR (quantitative polymerase chain reaction) |
| abstract | The invention discloses a method for determining the negative and positive RCR in a CAR-T cell product by detecting GALV and ALB copy number through multiple qPCR, which is characterized by comprising the following steps: and extracting DNA from the whole blood sample, synthesizing a plasmid containing a target gene and an internal reference gene to prepare a standard curve and a quality control sample, and establishing a multiple qPCR reaction system to detect RCR in the sample. The specificity is strong: specific probes are designed aiming at the target gene and pass the verification in the specific evaluation. The sensitivity is high: the reaction is more sensitive, and the detection lower limit of the method can reach 10 copies/mu L. Time and labor are saved: the method for detecting RCR by qPCR is simple to operate and easy to start, and the reaction time is only 1 hour. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116356005-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116356005-B |
| priorityDate | 2022-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.