http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114410750-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_850ce8560bc3deedfd3c0bba1573f8b5 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6825 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6825 |
| filingDate | 2022-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17518461ae60ef5a9829676b35022733 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_148e2e3cd615295fe32f16425fae90c6 |
| publicationDate | 2022-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114410750-A |
| titleOfInvention | Nucleic acid detection method and kit based on circular biped DNA walking strategy |
| abstract | The invention discloses a nucleic acid detection method and a kit based on a circular biped DNA walking strategy, wherein the kit comprises Probe A, Probe B, Probe D, Probe E, polymerase, nicking endonuclease, coenzyme factor, Tris-HCl, EDTA, TCEP, NaCl, mercaptohexanol, phosphate buffer solution, dNTPs and NEB buffer 2.1. The nucleic acid detection method and the kit based on the circular biped DNA walking strategy provided by the invention construct a reproducible DNA modified gold electrode interface, and the circular DNA structure constructed by the stem-loop structure and the dumbbell structure DNA under the catalytic reaction of target nucleic acid and related enzymes can perform the biped walking reaction of the electrode interface, so that the ultrasensitive quantitative analysis of the target nucleic acid can be realized, the detection limit can reach 2.2aM, and the kit has the characteristic of high selectivity, and can be well applied to nucleic acid analysis and detection. |
| priorityDate | 2022-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 94.