| abstract |
The neutralizing antibody of the present invention is obtained through screening single B cell flow sorting-antibody gene amplification pairing expression technology, has unique CDR region, can combine specifically with SARS-COV-2 and can neutralize the concerned variant strain (SARS-CoV-2 WT, alpha, Beta, Gamma, Delta, Omicron) in the world health organization, especially the IC50 of the neutralized Omicron variant strain is lower than 0.1 microgram/mL. The invention also relates to a preparation method and application of the group of neutralizing antibodies. The 4-strain antibody can be used for emergency prevention and/or treatment aiming at the global epidemic of the COVID-19 variant strain, has the characteristics of full humanization, high expression and good stability, and is suitable for industrialization. In addition, the antibody can also be used for preparing a SARS-COV-2 virus detection reagent, finding an effective neutralizing epitope and developing SARS-COV-2 recombinant protein and subunit vaccine. |