abstract |
The invention provides an N-methyl-D-aspartate receptor recombinant antigen, a preparation method thereof and a kit containing the recombinant antigen. The recombinant antigen comprises subunit GluN1a recombinant protein, wherein the amino acid sequence of the recombinant protein is that Asn61, Asn350, Asn471 and Asn771 in the sequence shown in SEQ ID NO. 1 are mutated into Gln, Cys22 is mutated into Ser, Glu595 and Glu597 are mutated into Ser, and Glu598 is mutated into Thr. The N-methyl-D-aspartate receptor recombinant antigen also comprises a natural subunit GluN2A recombinant protein and/or a subunit GluN2B recombinant protein, and the amino acid sequence of the N-methyl-D-aspartate receptor recombinant antigen is that Asn348 in the sequence shown in SEQ ID NO. 3 is mutated into Asp, and Cys838 and Cys849 are mutated into Ser. |