| abstract |
The invention provides a method and application for characterizing bacterial DNA methylation based on Nanopore sequencing technology, belonging to the field of biological information technology, comprising the following steps: obtaining Nanopore sequencing fast5 and fastq data of a target strain, and performing quality control on the sequencing data; Obtain the reference genome of the target strain, and use nanopolish or toombo software to obtain genome-wide DNA methylation levels of different modification types; control mechanism. The method provided by the invention can help to comprehensively characterize the DNA methylation map of bacteria, explore the previously hidden epigenetic regulation mechanism in the field of bacteria, and has important application value. |