http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114350707-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2022-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114350707-B |
| titleOfInvention | A method for efficient transient transfection of plasmids in carp EPC cells |
| abstract | The invention discloses a lactalbumin carrier used for transfection of carp EPC cells. The lactalbumin carrier is α-lactalbumin spherical particles whose surface is modified with maleimide. The present invention also discloses a preparation method of lactalbumin carrier, which comprises the following steps: making α-lactalbumin into spherical particles by means of ultrasonic repolymerization after enzymatic hydrolysis; connecting maleimide to the surface of α-lactalbumin particles to make α-lactalbumin particles It forms the lactalbumin carrier. The invention also discloses a method for efficient transient transfection of plasmids in carp EPC cells, comprising: mixing the lactalbumin carrier and the plasmid to be transfected, and adding them to the EPC cell culture medium to form a transfection culture medium; using the transfection culture medium After culturing the carp EPC cells for a period of time, the transfection medium was removed and replaced with the EPC cell medium to continue culturing. The invention has low cytotoxicity, simple operation and high transfection efficiency, can be used as a general gene introduction method for fish cells, and has good application prospects. |
| priorityDate | 2022-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.