http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114317825-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0d21d728fa50fd89fdda21942ee00057 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
filingDate | 2021-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4164e9ed5a26c21ab12c69d77a9e601a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_895e9ff4bb6eb04c4cacc22540c1b570 |
publicationDate | 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-114317825-A |
titleOfInvention | qPCR (quantitative polymerase chain reaction) diagnosis method for porcine reproductive and respiratory syndrome |
abstract | The invention discloses a qPCR (quantitative polymerase chain reaction) diagnosis method for porcine reproductive and respiratory syndrome, which is characterized by comprising the following steps of: the related reagents are primers, fluorescent probes, quantitative reagents and serum diluent, and the method comprises the following steps of collecting a sample; step two, adding a primer; adding a fluorescent probe; step four, sample amplification; step five, detecting by a fluorescent quantitative PCR instrument; step six, serum dilution is compared; step seven, detecting by a diluted serum PCR instrument; and step eight, comparing and analyzing the data. The invention utilizes the real-time fluorescent quantitative PCR method to measure the content of the virus nucleic acid in the blood, so that the antibody does not need to be generated in the pig body, and the antibody can be measured at the early stage of virus infection, namely when the virus content in the blood is very low, thereby not only improving the accuracy of the diagnosis of the porcine reproductive and respiratory syndrome, but also carrying out prediction and judgment on the amplification of the virus, providing a certain thought for the treatment at the later stage, and playing a role in promoting the prevention and control work of the porcine reproductive and respiratory syndrome virus. |
priorityDate | 2021-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.