http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114317523-A

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
filingDate 2021-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd865a8ad13f5da2ec5427d01de003a1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_735770f785fee1fd514d178e02684ace
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publicationDate 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114317523-A
titleOfInvention Extraction reagent and extraction kit for high-purity nucleic acid
abstract The application relates to the technical field of nucleic acid extraction, and particularly discloses an extraction reagent and an extraction kit for high-purity nucleic acid. The extraction reagent comprises lysis solution, magnetic bead solution, binding solution, washing solution A, washing solution B and eluent; the lysis solution comprises the following components: 15-25 mmol/L Tris-HCl with the pH value of 7.8-8.2, 0.5-1.2 mol/L guanidinium, 0.05-0.25 mol/L polyhexamethylene guanidine, 0.8-1.5 mol/L sodium salt, 0.3-0.8 wt% sodium sarcosinate, 0.1-2.5 wt% polyvinylpyrrolidone, 0.1-5 wt% cationic surfactant, 5-14 mmol/L enzyme inhibitor and water as a solvent; the extraction kit contains the extraction reagent. The extraction reagent can be used for nucleic acid extraction, and has the advantages of high purity and high concentration of extracted nucleic acid.
priorityDate 2021-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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