http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114317523-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bfb5863c92ccb8cbd10531fa3d154ac9 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2021-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd865a8ad13f5da2ec5427d01de003a1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_735770f785fee1fd514d178e02684ace http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_053ebff02c6734396fc2c290df256bdc |
publicationDate | 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-114317523-A |
titleOfInvention | Extraction reagent and extraction kit for high-purity nucleic acid |
abstract | The application relates to the technical field of nucleic acid extraction, and particularly discloses an extraction reagent and an extraction kit for high-purity nucleic acid. The extraction reagent comprises lysis solution, magnetic bead solution, binding solution, washing solution A, washing solution B and eluent; the lysis solution comprises the following components: 15-25 mmol/L Tris-HCl with the pH value of 7.8-8.2, 0.5-1.2 mol/L guanidinium, 0.05-0.25 mol/L polyhexamethylene guanidine, 0.8-1.5 mol/L sodium salt, 0.3-0.8 wt% sodium sarcosinate, 0.1-2.5 wt% polyvinylpyrrolidone, 0.1-5 wt% cationic surfactant, 5-14 mmol/L enzyme inhibitor and water as a solvent; the extraction kit contains the extraction reagent. The extraction reagent can be used for nucleic acid extraction, and has the advantages of high purity and high concentration of extracted nucleic acid. |
priorityDate | 2021-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.