| abstract |
The invention discloses a construction method of a muscle cell line of Gymnocypris przewalskii, which comprises primary culture, subculture, cell cryopreservation and recovery, wherein the cell culture solution of the primary culture comprises the following components: a basic culture medium, 10-30% of bovine serum, 200-400 IU/mL of penicillin, 200-400 mug/mL of streptomycin, 1-5 mmol/L of glutamine and 1-5 mmol/L of sodium pyruvate; the components of the subcultured cell culture solution comprise: a basic culture medium, 10-30% bovine serum, 50-250 IU/mL penicillin, 50-250 mug/mL streptomycin, 1-5 mmol/L glutamine and 1-5 mmol/L sodium pyruvate; the frozen stock solution for freezing the cells comprises the following components: a basic culture medium, 10-30% of bovine serum, 50-250 IU/mL of penicillin, 50-250 mug/mL of streptomycin, 5-30% of DMSO, 1-5 mmol/L of glutamine and 1-5 mmol/L of sodium pyruvate. The preparation method is simple, does not need special equipment, can be operated in a common sterile culture room, and the prepared cells have good and stable growth and can be used for researches on nutrition, immunity, environmental toxicity, gene function analysis and the like. |