http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114315979-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 |
| filingDate | 2021-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114315979-B |
| titleOfInvention | Light-cleavable protein mutant with high light-cleavage efficiency and application thereof |
| abstract | The invention relates to the technical field of genetic engineering, and discloses a photocleavable protein mutant with high photocleavage efficiency and application thereof. The mutant of the photo-cleavable protein is a mutant of photo-cleavable protein phoCl, the amino acid sequence of the photo-cleavable protein phoCl is shown as SEQ ID NO:1, and the mutant of the photo-cleavable protein has mutation in at least one of the 8 th to 232 th amino acid residues of the photo-cleavable protein phoCl. The photocleavable protein mutant provided by the invention can obtain higher cleavage efficiency in shorter UV illumination time. The photocleavable protein mutant provided by the invention can be fused and expressed with different target proteins, the obtained fusion protein has high purification efficiency, and the high-efficiency recovery of the target protein is realized. |
| priorityDate | 2021-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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