http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114304062-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K49-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-444 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K45-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P27-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P37-02 |
| filingDate | 2021-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114304062-B |
| titleOfInvention | A method for constructing an animal model of autoimmune retinopathy and its application |
| abstract | The present invention relates to a method for constructing an animal model of autoimmune retinopathy, which is characterized in that it comprises the following steps: (1) adding recombinant mouse Recoverin protein to an equal volume containing 4 mg of heat in an amount of 1-3 mg/ml In the incomplete fluorine adjuvant of inactivated Mycobacterium tuberculosis H37Ra, mix it with a homogenizer at a speed of 8000-10000rpm/min at 0-4°C for 1-2h, and then repeatedly pump it into a 5ml syringe until it is mixed. Homogenize into an emulsion and the emulsion does not disperse; (2) Take 8 7‑9 week old C57BL/6 male or female mice and divide them into two groups at random: Experimental group: Subcutaneously placed on the upper back of the mouse, according to the content of recombinant recoverin protein Inject the emulsion prepared in step (1) at an amount of 200-300 μg/mouse, and inject pertussis (PTX) into the abdominal cavity of mice on the 0th and 2nd days of immunization at the amount of 200ng/mouse; control group: no Inject the incomplete fluorine adjuvant emulsion containing recombinant recoverin protein and heat-inactivated Mycobacterium tuberculosis H37Ra; (3) Observe the mice in the experimental group and the control group in the 3‑8th week to verify the activation of microglial cells Mechanisms leading to autoimmune retinopathy. |
| priorityDate | 2020-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.