http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114292900-A

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
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filingDate 2021-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfc29b1d40637da1be84f70043da114d
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publicationDate 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114292900-A
titleOfInvention Probe mixing proportion design method and mixed probe set and sequencing library construction method
abstract A probe mixing proportion design method and a mixed probe set and sequencing library construction method are provided, the probe mixing proportion design method comprises the following steps: the pre-estimating step comprises providing probe sets, and pre-estimating the mixing proportion of the probe sets according to the chip size of each probe set and the sequencing depth requirement; and the step of predicting the sequence, which comprises the steps of setting a premixing proportion according to the estimated mixing proportion, mixing the probe set according to the premixing proportion to obtain a premixed probe, hybridizing the premixed probe with the sublibrary, and performing pre-sequencing to obtain the probe mixing proportion which is in line with the expectation. According to the invention, probes of a plurality of different target areas can be respectively designed according to the product requirements, and mixed probe preparation can be carried out according to the sequencing depth requirements, so that the purpose of simultaneously carrying out different sequencing depth detection on different target areas is achieved, and the cost is low; the probe proportion can be adjusted according to the change of the product target, or different probe combination modes can be detected, and the flexibility is high.
priorityDate 2021-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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Total number of triples: 28.