http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114292900-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b837781998e4e578680acb9230cf23a1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_432d49e6f209dca7b49cccb7ae17ed22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bfd32ea576c54943be377c7bab783afb |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
filingDate | 2021-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfc29b1d40637da1be84f70043da114d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0001f638485eb92301578a3b2b44fda2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aac479919a7e274b7f407cb30bc966c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4683288969badb20b182c1593e386051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_525b63ecafe6d1a03796a230abb65546 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5ffa6998376cf1f2de1f733850974d7 |
publicationDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-114292900-A |
titleOfInvention | Probe mixing proportion design method and mixed probe set and sequencing library construction method |
abstract | A probe mixing proportion design method and a mixed probe set and sequencing library construction method are provided, the probe mixing proportion design method comprises the following steps: the pre-estimating step comprises providing probe sets, and pre-estimating the mixing proportion of the probe sets according to the chip size of each probe set and the sequencing depth requirement; and the step of predicting the sequence, which comprises the steps of setting a premixing proportion according to the estimated mixing proportion, mixing the probe set according to the premixing proportion to obtain a premixed probe, hybridizing the premixed probe with the sublibrary, and performing pre-sequencing to obtain the probe mixing proportion which is in line with the expectation. According to the invention, probes of a plurality of different target areas can be respectively designed according to the product requirements, and mixed probe preparation can be carried out according to the sequencing depth requirements, so that the purpose of simultaneously carrying out different sequencing depth detection on different target areas is achieved, and the cost is low; the probe proportion can be adjusted according to the change of the product target, or different probe combination modes can be detected, and the flexibility is high. |
priorityDate | 2021-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.