| abstract |
The present invention relates to methods of producing lentiviral vector producing cells. Specifically, the method utilizes two plasmids (rather than four) to provide cells with the required packaging elements and transfer vectors, thereby generating large numbers of lentivirus-producing cells, including suspension cells, and producing large numbers of lentiviruses. These methods generate cells that can then be induced to produce lentiviruses that, by tailoring, contain specific target genes. |