http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114264822-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_87a2158ed8d025c91f43b30bdca192f9 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2020-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63ddcd12ca45630b1b6871a5484a4a14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_873f930b5dc5035e1c32feaeba648057 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d47fa7e9db1876a0b3e61e20f15c869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_445c17d43cfb8888b76e233f6fad143a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e31599d3239578aac1067cc9d1331d87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_567d7c1bf76f5f6f416afaf4308734ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67c1dffb55046c45d95503cb7cfb58a4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d70d690d8f32550ec9ae51d6132f5971 |
| publicationDate | 2022-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114264822-A |
| titleOfInvention | Method for detecting absolute content of nicotinamide and nicotinamide mononucleotide |
| abstract | The invention provides a method for detecting absolute contents of Nicotinamide (NAM) and Nicotinamide Mononucleotide (NMN), which can quickly, simply and conveniently detect the absolute contents of NAM and NMN and belongs to the technical field of molecular biology. Firstly, selecting the concentration of an NAM standard substance, processing the NAM standard substance and a sample at the same time, and making an NAM standard curve during data analysis; separating, cracking and/or blocking the intrinsic enzyme reaction of the sample; then, adding excessive Methyl Nitrosourea (MNU), acetophenone, KOH and formic acid into the standard substance, the sample and the blank in sequence for reaction to finally obtain the fluorescent derivative; detecting the fluorescence intensity by using an enzyme-labeling instrument; and finally, analyzing data, and calculating the NAM and NMN content of the sample according to the standard curve. The detection method has the advantages of simple operation, low cost, accuracy, rapidness, short time consumption and high flux. |
| priorityDate | 2020-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 54.