http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114250239-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be2c55a9de09e08e3404c0a5a1ae4dc7 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-32 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2021-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6168fa259c6d39ae30db50e0a9290df http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ad586c616d8b9cde395f0f8dd9edfe6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0fc184eb7fa0aa6d87bc10bbc667bbbc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e881f4c020ebc704679d59e9e91f7f4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33c9aad24052bdba2451622a3f0253f5 |
| publicationDate | 2022-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114250239-A |
| titleOfInvention | Construction method and application of glycine riboswitch gene regulation circuit |
| abstract | The invention relates to a construction method and application of a glycine riboswitch gene regulation circuit; through mutation ON a wild type glycine riboswitch element sequence, glycine-ON mutants with improved performance, increased glycine response range and increased amplitude value and glycine riboswitch mutants with changed conformation are screened out from a library at high flux, so that the expression regulation of genes can be realized. The invention simultaneously converts wild glycine-ON into glycine-OFF riboswitch and increases the amplitude value of the riboswitch. The invention also provides a method for screening glycine mutants with improved performance and changed conformation, and an expression vector and a host cell containing the mutants. The invention discloses a glycine riboswitch gene regulation circuit construction, performance improvement and conformation change mutant, which is an effective mutant based on a glycine riboswitch circuit and performance improvement reported for the first time in escherichia coli at present. |
| priorityDate | 2021-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 366.