| abstract |
The present invention provides a nanoparticle comprising a scaffold, a single template site for binding a template polynucleotide to the scaffold, and a plurality of helper sites for binding a helper oligonucleotide to the scaffold, wherein the scaffold is selected from one or more scaffold DNA molecules and one or more scaffold polypeptides, the single template sites for binding the template polynucleotide to the scaffold are selected from covalent template binding sites and non-covalent template binding sites, And the plurality of helper sites for binding the helper oligonucleotide to the scaffold are selected from covalent helper oligonucleotide binding sites and non-covalent helper oligonucleotide binding sites. Also provided are methods of using nanoparticles, particularly in sequencing by synthesis. |