| abstract |
The invention discloses an SSR core primer group for identifying the purity of cauliflower hybrids and a screening method and application thereof, belonging to the technical field of molecular markers. The SSR core primer group comprises: BoGMS0624, OI11G11, BoGMS 0941; the alternative core primer group is as follows: BoE607, BoE761, BoGMS2431, BoGMS0808 and BoGMS 1530. According to the method, through DNA fingerprint analysis of 70 hybrid species of cauliflower and broccoli, the evaluation index of the core primer suitable for purity identification of the hybrid species is determined, a set of primers for purity identification of the cauliflower hybrid species is determined, the purity identification of the cauliflower hybrid species is realized by the least primers with wide genome coverage, the workload is greatly reduced, the detection efficiency is improved, and the detection cost is reduced. |