abstract |
The invention discloses an oncolytic adenovirus recombinant carrying TMTP1 and HSV-TK, a construction method and application thereof, and belongs to the technical field of medical genetic engineering. The invention deletes 27 bases of 920-946nt region in E1A conserved sequence 2 region of human 5-type adenovirus gene, inserts gene sequence coding tumor targeting peptide TMTP1 in 19641-19655nt region of Hexon hypervariable region 5, simultaneously deletes E3 region in 29477-29714nt region of ADP gene to form deleted region, inserts full length coding sequence coding HSV-TK gene in the deleted region and introduces Cla1 enzyme cutting site. The invention also discloses a construction method and application of the oncolytic adenovirus recombinant. The oncolytic adenovirus recombinant carrying TMTP1 and HSV-TK has ideal targeting effect and strong killing effect. |