| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_73aec6ea0f83ed93e7f17fd860893c61 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-501
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4703
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-241
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0682
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-78 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate |
2021-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_36ba19399f0e45a67df52526977e7cf2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0cef6eef97e64d82882d00c1ae2b4098
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df464701cc7fad510761365304312db9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73f260d3ccc698e878a3851c5df18f0f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f4c70846341a51ac53970b0da669b55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b02cb144cac0e444b1a522f35802326a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fdba2a73863713381d72b7ba48308f9a |
| publicationDate |
2022-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-114214359-A |
| titleOfInvention |
A method for improving the expression level of recombinant protein in CHO cells |
| abstract |
The invention belongs to the technical field of genetic engineering, and in particular relates to a method for improving the expression level of a CHO cell recombinant protein. It comprises the following steps: (1) Co-transfecting CHO cells with the recombinant vector of the target gene and the co-transgenic recombinant vector containing the cell cycle regulation-related gene p18 and/or the gene aFGF that regulates cell growth; the sequence of the gene p18 is as shown in SEQ ID NO. : 1; the sequence of the gene aFGF is shown in SEQ ID NO: 2; (2) after the co-transgenic recombinant vector and the target gene recombinant vector are co-transfected into CHO cells, cultured and screened to obtain the expression of CHO cells (3) Suspension and serum-free culture of transiently and stably transfected CHO cell lines to obtain the target protein. The method of the invention can significantly improve the expression level of the target gene in the CHO cell, and realize the transient and stable high-level expression of the target protein in the CHO cell. |
| priorityDate |
2021-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |