http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114200065-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-74 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-74 |
| filingDate | 2021-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114200065-B |
| titleOfInvention | Method for Quantitative Determination of In Vitro/In Vitro Superoxide Radical Content Generated by Environmental Stress |
| abstract | The invention provides a method for quantitatively measuring the content of superoxide radicals in vivo/external generated by environmental stress, which comprises the following steps: step S1, preparing a test solution: taking a sample to be tested, and preparing a test solution; Step S2, prepare a reference substance solution: take the standard substance 2,7-dichlorofluorescein, dissolve it in water, and prepare a reference substance solution; Step S3, use a high performance liquid chromatograph-fluorescence detector to analyze the test solution and the reference substance solution. In step S4, take the peak area of the reference solution as the ordinate and the concentration of the reference solution as the abscissa, draw a standard curve, and calculate the 2,7-dichlorofluorescein in the test solution according to the standard curve the content of superoxide radicals. By adopting the technical scheme of the present invention, ROS can be quantitatively measured, the interference of external environmental factors is reduced, the comparability between different batches is made, and the method is simple and reliable. |
| priorityDate | 2021-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 243.