| abstract |
The invention discloses an HPLC detection method for content of related substances in p-bromoanisole, which comprises the steps of respectively injecting sample solution and reference solution, and detecting the content of p-bromophenol and o-bromoanisole in p-bromoanisole by an HPLC method, wherein the HPLC chromatographic conditions are as follows: a chromatographic column: octadecyl bonded silica gel chromatographic column; mobile phase: taking 0.01mol/L sodium dihydrogen phosphate solution as a mobile phase A and acetonitrile as a mobile phase B; carrying out elution; column temperature: 25-40 ℃; flow rate: 1.0 ml/min; sample introduction volume: 1-20 mul; a detector: UV detector: 210-230 nm. The HPLC detection method has good system applicability, detection limit, quantitative limit, durability, precision, accuracy and recovery rate, is beneficial to the quality control of the bromoanisole, and can better ensure the quality of the N-aryl pyridone drugs. |