http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114196656-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a78e020de49fa4759c25eb9b8d0be208 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-102 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57 |
| filingDate | 2021-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51a99cbbda40e59cca48bdc554921dbe |
| publicationDate | 2022-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114196656-A |
| titleOfInvention | Protease K mutant and construction and application of expression vector thereof |
| abstract | The invention discloses a protease K mutant and construction and application of an expression vector thereof, relating to the technical field of biological engineering, and providing the protease K mutant which has at least one of the following mutations on the basis of an amino acid sequence shown in SEQ ID No. 1: F371C and T373P have better enzyme activity compared with wild type proteinase K, and provide a way for the application and research of proteinase K. In addition, the invention also provides the construction of the proteinase K mutant high-copy expression vector, improves the screening weighing power of high-expression thallus, reduces the operation of screening a large number of bacteria, and indicates the direction for the industrial production route of the proteinase K with application value. |
| priorityDate | 2021-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 227.