| abstract |
The application relates to a culture medium for efficiently culturing hematopoietic stem cells in vitro, belonging to the technical field of cell in vitro culture. The culture medium for efficiently culturing the hematopoietic stem cells in vitro comprises a basic culture medium and an additive factor; the basic culture medium is a serum-free culture medium; recombinant human stem cell factor: 50-200ng/ml, recombinant human thrombopoietin: 20-100ng/ml, recombinant human FMS-like tyrosine kinase 3 ligand: 100-200ng/ml, interleukin-3: 30-60ng/ml, polyvinyl alcohol: 1-10mg/ml, insulin-transferrin-selenium-ethanolamine additive: 1-2:100ml/ml, indole analogue: 10-200ng/ml, nitrogen heterocyclic compound: 10-200 ng/ml. The defects of low expansion rate, easy differentiation and the like of the hematopoietic stem cells in the prior art are overcome; the proliferation efficiency of the hematopoietic stem cells is improved by 50 to 100 times, and the number of the obtained hematopoietic stem cells is more. |