http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114196611-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-14 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00 |
| filingDate | 2021-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114196611-B |
| titleOfInvention | Application of a buffer stabilizer containing ectoine in the preparation of protoplasts |
| abstract | The invention relates to the technical field of protoplast preparation, in particular to the application of a buffer stabilizer containing ectoine in the preparation of protoplasts, wherein the buffer stabilizer contains ectoine with a final concentration of 0.02-0.2 mol/L and MgCl 2 with a final concentration of 0.1 to 0.2 mmol/L. In the invention, the small molecule amino acid derivative ectoine is added to the buffer stabilizer, which can protect cells from damage under extreme conditions such as high salt and high temperature, and has the functions of stabilizing osmotic pressure, protecting enzymes, DNA, proteins and biological membranes ; And the buffer stabilizer used is suitable for the protoplast preparation process of plants and microorganisms, which can reduce the damage of protoplasts in the preparation process, achieve more complete protoplasts and prolong the storage time of protoplasts, and maintain the activity for a long time. Unbreakable purpose. |
| priorityDate | 2021-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.