| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ed45040c25720954487cd2e14c16052a |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P25-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1247
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07006 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P25-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 |
| filingDate |
2021-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae47418fdd25be9e588577c65d628ce8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8aa17e8e511e24857e8812452f236a0f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5968c2ff230ec499eec30f4beffefa74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6be75cc3093d978224a447e438c4c062
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9eefc3125a12c55af8cb183ff0ffe3b8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ebf45e60a9d71680fe7ef4f16ecf05e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c5018d06a6bb7b872386e42e1b5a119
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fee54dcf36b3dd03307a3a7be2e2e6c4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ab4ae8c16151506c57a378b1422b1da
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f825731c698f4c3874d14b2ad61e5d83
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac7b469914c86271235b4cc064bb5abe |
| publicationDate |
2022-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-114181963-A |
| titleOfInvention |
A method for improving riboflavin-producing ability of Escherichia coli engineering bacteria by using DNA shuffling |
| abstract |
The invention provides a method for improving the riboflavin-producing ability of Escherichia coli engineering bacteria by using DNA shuffling, and relates to the field of genetic engineering, comprising the following steps: constructing a riboflavin-producing Escherichia coli engineering bacteria containing 26 genes; T7RNA polymerase The first batch of positive plasmids were obtained by DNA rearrangement and directional screening of gene expression units; the second batch of positive plasmids were obtained by DNA rearrangement and directional screening of riboflavin biosynthesis and transport system; After DNA rearrangement and directional screening, the third batch of positive plasmids was obtained by extraction; the third batch of plasmids were introduced into different chassis cells to obtain recombinant strains; the positive strains with the highest riboflavin production were selected. Effectively eliminate the toxicity of T7RNAP to the E. coli host, the feedback inhibition of the product and the moderate and coordinated expression of the constructed riboflavin biosynthesis gene during the construction of riboflavin-producing E. coli engineering bacteria. Further improve the efficiency and yield of riboflavin biosynthesis by Escherichia coli engineering strains. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114806987-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114854780-A |
| priorityDate |
2021-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |