Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7a44789744c85216dc4636a64b0b6b77 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-18034 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-55555 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-18022 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-16134 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-16122 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-055 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-6931 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P11-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-155 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-065 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06 |
filingDate |
2021-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f55155a4f00b6c55170b06c140dbaf8 |
publicationDate |
2022-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-114181316-A |
titleOfInvention |
Self-assembled protein nanoparticles and uses thereof |
abstract |
Self-assembled protein nanoparticles (SAPN) are excellent antigens because they are capable of presenting multiple epitopes to B cells simultaneously and produce stronger B cell receptor signaling than a single epitope. Most SAPNs are derived from the capsid proteins of viruses or bacteriophages, with low particle stability, the presence of antibodies against the capsid proteins and structural incompatibility with peptide insertion. In the present invention, the inventors created SAPN using non-viral proteins that are thermostable and resistant to incorporation of target peptides. The assembled subunit of SAPN is a fusion protein between two components: a first component, a polymeric moiety consisting of an amphipathic helical peptide resulting from modification of influenza a virus M2 protein, and a second component, a target peptide presentation moiety consisting of a superfolder green fluorescent protein (sfGFP) having a peptide insertion site in a specific loop of sfGFP. The particles are capable of incorporating a target peptide by genetic recombination and presenting the target protein on the surface of the nanoparticle, stimulating the production of high affinity antibodies to the target peptide without the use of adjuvants. |
priorityDate |
2020-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |