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publicationDate 2022-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114166973-A
titleOfInvention Rapid quantitative detection method for aflatoxin B1 in vegetable oil
abstract The invention discloses a method for quickly and quantitatively detecting aflatoxin B1 in vegetable oil, which comprises the following steps: weighing a proper amount of vegetable oil sample in a centrifuge tube, adding a methanol aqueous solution, uniformly mixing by vortex, extracting by oscillation, centrifuging, and taking supernatant for later use; transferring the supernatant, adding water to dilute twice, transferring to an immunoaffinity column for purification, leaching the affinity column twice with water after the sample liquid is dripped, draining the affinity column by a vacuum pump, eluting by 1mL of methanol, collecting all the eluates, transferring 0.5mL of the eluates, diluting to a constant volume of 1mL with water, and mixing uniformly to obtain a solution to be detected; and detecting the liquid to be detected by adopting a high performance liquid chromatography-post column derivatization method, and calculating the content of the aflatoxin B1 in the sample. The method simplifies the processes of sample extraction, sample loading solution preparation, purification and detection, greatly simplifies the experimental steps, improves the working efficiency, has the characteristics of high selectivity, high sensitivity and accurate qualitative and quantitative analysis, and is suitable for quantitative analysis of aflatoxin B1 in vegetable oil.
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