| abstract |
The invention relates to a construction method of engineering bacteria for preparing ephedrine and a preparation method of the ephedrine, wherein the construction method comprises the following steps: the recombinant plasmid containing the hydroxymandelate synthase gene Hmas and the acetohydroxy acid synthase gene AHAS and the recombinant plasmid containing the mandelate dehydrogenase gene SMDH and the benzoyl formate decarboxylase gene BFD are transformed into escherichia coli or saccharomyces cerevisiae. The plasmid-based expression engineering bacteria constructed by the method can realize biosynthesis and ephedrine preparation, have industrial application prospects of large-scale production, high yield, environmental friendliness and the like, can obtain more stable expression strains by integrating corresponding gene modules through genomes in the future, and can further improve the yield by feeding phenylalanine, benzaldehyde and other feeding modes. |