http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114134109-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_241aa6790d0f27575e35ae46f0549fdd |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0662 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate | 2021-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5a09fb4c373eda43d688e5a22de97e9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1905969d24f7b6715cfe9a99feea6a6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4cd18d68bedf49f92e37116c61b66919 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d38d14d90f1d89e82a249fd9d18dafa4 |
| publicationDate | 2022-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114134109-A |
| titleOfInvention | Purification method of exosomes from EGF mesenchymal stem cells |
| abstract | The invention relates to a method for purifying EGF mesenchymal stem cell exosomes, comprising the following steps: Step 1: centrifuge a cell culture liquid containing EGF mesenchymal stem cell exosomes, and take the supernatant; The supernatant collected in step 1 is filtered and concentrated by a tangential flow ultrafiltration system to obtain a concentrate; step 3: the concentrate in step 2 is separated and purified by an anion exchange chromatography column, and the elution peak solution is collected; step 4: the The elution peak solution collected in step 3 is separated and purified by a heparin affinity chromatography column, and the elution peak solution is collected to obtain the EGF mesenchymal stem cell exosomes. This method can specifically separate and purify EGF mesenchymal stem cell exosomes, and can effectively separate exosomes carrying EGF protein and those without EGF protein. Compared with the traditional ultracentrifugation method, the extracted EGF mesenchymal stem cell exosomes are more EGF protein content is higher, and can maintain the integrity and biological function of exosomes. |
| priorityDate | 2021-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 172.