| abstract |
The invention discloses a preparation method and application of a secreted autophagosome surface protein detection chip. The preparation method of the chip is as follows: according to a designed mask, a shuttle-shaped array microfluidic chip is prepared by a soft lithography method, and biotin The biotinylated gelatin solution and avidin solution are alternately passed into the chip and left to stand, so as to realize the layer-by-layer assembly of the chip surface; the biotinylated capture antibody LC3B can be modified on the assembled chip surface to obtain microfluidic control chip; the invention also prepares detection antibody probes modified with different quantum dots for the detection of surface proteins of secreted autophagosomes; the invention has the advantages of less sample consumption, rapid and simple detection, good selectivity, high sensitivity, high-pass The advantages of high volume, good stability, etc., can achieve the purpose of related cancer diagnosis. |