http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114107483-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b7cea66c51951e4ab3c3c2c435464bf0 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-90251 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-558 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6893 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54313 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-558 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 |
filingDate | 2021-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-114107483-A |
titleOfInvention | Preparation method of FTO demethylase detection kit and detection kit |
abstract | The application discloses a preparation method of an FTO demethylase detection kit, the detection kit and application. The preparation method comprises the following steps: preparing a sample pad: soaking a nitrocellulose membrane in phosphate buffer solution containing 2% BSA, 0.1MNaCl and a surfactant at 4 ℃ for 8 hours, and drying to obtain a sample pad; treating the filter membrane; preparing a bonding pad: respectively marking fluorescent microspheres with antibodies containing 4 sites of FTO demethylase, adding a microsphere diluent, and uniformly mixing to obtain an antibody mixed solution; uniformly paving the antibody mixed solution on the treated sample pad, and carrying out vacuum freeze drying and sealing to obtain a bonding pad; preparing a chromatographic membrane: respectively preparing a detection band and a quality control band on the nitrocellulose membrane to obtain a chromatographic membrane; preparing absorbent paper; and (6) assembling. The FTO demethylase kit disclosed by the invention is beneficial for obese high-risk persons to know the self genetic condition in advance, and obesity and overweight are avoided by adjusting the dietary structure and the life style in advance. |
priorityDate | 2021-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 76.