| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d09264768c6247093f1e0f62b63dc143 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-9122
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56933
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-065
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-01021
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1211
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate |
2021-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_845a3cea0a69bf51c4ba074641471699
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_46add202feda3e85a8d1061e3326c29f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81644cf779997905ec676221a09003a1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_633d98e21d95a02068fbe90e12db94d6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5656779a2793f517aa664fdfd8e8725e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f611936c7c32d294e381b032e0997b1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58f0e91972f765d1d6007ab9af5d8749 |
| publicationDate |
2022-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-114107339-A |
| titleOfInvention |
Paulownia arbuscular phytoplasma thymidine kinase gene primer, gene, fusion protein, polyclonal antibody and application thereof |
| abstract |
The invention provides an amplification primer of a thymidine kinase gene of paulownia arbuscular phytoplasma, and two genotypes are obtained by amplification; constructing a prokaryotic expression vector of thymidine kinase of paulownia arbuscular phytoplasma, and performing induced expression and purification to obtain 6 XHis/PaWB TDK-1 fusion protein and 6 XHis/PaWB TDK-2 fusion protein; rabbit polyclonal antibody is obtained by immunizing rabbit with fusion protein as immunogen; the polyclonal antibody can generate immunoreaction with both PaWB TDK-1 protein and PaWB TDK-2 protein; by means of immunoblotting, immunofluorescence microscopy and immunoelectron microscopy, the polyclonal antibody is proved to be better used for detecting the paulownia arbuscular phytoplasma and thymidine kinase expression thereof. The invention lays a foundation for further researching the multiplication pathogenesis of phytoplasma and provides a new technical means for detecting the phytoplasma of paulownia arbuscular branches in agriculture and forestry production. |
| priorityDate |
2021-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |