| abstract |
The invention discloses a recombinant turkey herpes virus live vector vaccine which simultaneously expresses the VP2 protein of classical strain and variant strain infectious bursal virus, and belongs to the field of veterinary biological products. In the present invention, the VP2 protein and LTB fusion expression cassette containing the classic strain of infectious bursal virus is knocked into the non-essential region of US2 virus replication of turkey herpes virus, and the VP2 protein and LTB fusion expression cassette of the variant strain is inserted into the US10 virus replication non-essential zone. The recombinant virus finally obtained can efficiently express the LTB-VP2 fusion antigen protein of IBDV classic strain and variant strain at the same time in CEF cells. The vaccine prepared by the invention can improve the antibody level after immunization, improve the uniformity of the antibody after immunization, and ensure the immunization effect of the vaccine. The vaccine has the advantages of high efficiency, good safety and lifetime immunity after one-time inoculation, and will not cause clinical reactions to chicks. and pathological damage, the vaccine protection rate reached 100%. |