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filingDate 2021-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b6735d374eaec4ac65f61ff47b4e154
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publicationDate 2022-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114088861-A
titleOfInvention Method for detecting enterotoxin C in milk by using multi-dimensional liquid chromatography-mass spectrometry
abstract The invention discloses a method for detecting enterotoxin C in milk by using a multi-dimensional liquid chromatography-mass spectrometry, which comprises the following steps: step S1: selecting a two-dimensional separation mode, and constructing a two-dimensional liquid chromatography platform comprising a first-dimensional ion exchange liquid chromatography and a second-dimensional reversed-phase liquid chromatography; step S2: and separating the milk by the two-dimensional liquid chromatography platform to obtain fractions, and identifying enterotoxin C by mass spectrometry identification of fraction complete protein and mass spectrometry identification of enzymolysis peptide. The method for detecting enterotoxin C in milk by using the multi-dimensional liquid chromatography-mass spectrometry combination provided by the invention has the advantages of accurate identification, rapidness, effectiveness, high sensitivity and the like. The method overcomes the defect of low accuracy of the existing ELISA method, has high automation degree, improves the detection flux, reduces the test cost, is convenient and accurate to judge the result and has higher practicability.
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