| abstract |
The invention discloses a novel coronavirus 4-point mutation S gene identification kit based on a high-resolution melting curve and an identification method thereof, which relate to the field of biomedicine and are characterized in that amplification primers are designed according to 4 different mutation sites of a novel coronavirus S gene, and the S gene with different mutation sites is identified through an optimized high-resolution melting curve reaction system. The kit has the advantages of simple operation, high reaction speed, no need of post-treatment of PCR products, operability and reproducibility, and can be used for identifying novel coronavirus S genes with different site mutations. |