http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114085870-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_653c418e41edbb4e1ce8bc5e53e70cec |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10043 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-861 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2021-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3dbed3f25a74c55e25db429351f2a9f9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_48fd490351fdaab12e7c0646164dd7bd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3f7788f55d6fc09ce1c52fb1b70ad57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7616ef0ff5aa355bf322dfeafc524f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_147edfb5afd242912fe436095ff669c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e464999caeb0af3bc13f52bc28efe94 |
| publicationDate | 2022-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114085870-A |
| titleOfInvention | A method for labeling live cells in the fascicular cortex of adrenal tissue |
| abstract | The invention provides an in vitro targeting method of adrenal tissue fascicular cortex living cell labeling, which belongs to the technical field of biomedicine. Treat the target plasmid with Pac I; transfect the cells with the Pac I-treated plasmid; add complete culture medium after transfection for a certain period of time; centrifuge, freeze the cells, and dissolve them in a water bath; spread the 293 cells in a culture plate, and add the virus-containing supernatant When there are cells floating off, collect and purify to obtain recombinant adenovirus; sorting of adrenal tissue fascicular cortex viable cells transfected with green fluorescent protein induced by adenovirus. The invention uses adenovirus for cell labeling, uses 293 cells as packaging cells, enriches virus particles through multiple amplification, separates and purifies through CsCl gradient centrifugation and dialysis, improves the purification degree of cell sorting, and can be used on a large scale. Applied in adrenal tissue fascicular cortex live cell labeling technology. |
| priorityDate | 2021-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.